Planta Med. Jan;71(1) Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures. Wilhelmson A(1), Kallio. Plant Cell Rep. Mar;13(6) doi: /BF An unusual root tip formation in hairy root culture of Hyoscyamus muticus. Jaziri M(1), Homes . Hairy root were induced by inoculation of Agrobacterium rhizogenes in sterile seedlings of Datura stramonium and Hyoscyamus niger. The transformed roots.

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An unusual root tip formation in hairy root culture of Hyoscyamus muticus.

From This Paper Figures, tables, and topics from this paper. All of the Alkaloid Extraction and Analysis. Hairy roots were cryopreserved along a modified method essentially described for banana apical meristems by Panis et al. Cryoprotection on the other hand covers the use of permeable uairy which function through a colligative effect and usually result in the increase in membrane fluidity. Nuclear localization and interaction of RolB hsiry plant proteins correlates with induction of adventitious roots by the oncogene rolB.

Stability of Hairy Root Cultures Genetic stability of hairy roots is well recognized. In addition, hairy roots gain biomass rather rapidly and have simple cultivation medium requirements. For organized tissues mutkcus as hairy root apical meristems, the labor intensive cutting and handling of small and fragile tissue parts is a clear obstacle for high-throughput testing.

Showing culyure 48 extracted citations. Academic, New YorkVol. PCR was performed using h6h gene-specific primers, and it was shown that both transgene and root phenotype responsible rolB region were present in H.

Agrobacterium Rhizobiaceae is a soil bacterium, which is able to deliver a part of its own plasmid-DNA T-DNA into the nuclear genome of the plant cell.

Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures.

Topics Discussed in This Paper. This means that you will not need to remember your user name and password in the future and you will be able to login with the account you choose to sync, with the click of a button.


Several hyoscyamine-rich but scopolamine-poor plants exploration of metabolic engineering as a potential effective that had been considered unattractive for commercial exploita- approach to increase the yield of specific metabolites by enhanc- tion may now become promising candidates for large-scale ing rate-limiting steps or by blocking competitive pathways. So far, explants of H. The major constraint with undifferentiated cell cultures is that they are generally considered to be genetically unstable and cultured cells tend to produce low yields of secondary metabolites especially over time.

Towards industrial usefulness — cryo-cell-banking of transgenic BY-2 cell cultures. Approximately 10 tips were placed on the cryo-vial together with 1 ml LSP solution 2 M glycerol, 0.

The first analyses were performed using immunoassays while later the alkaloids were analyzed by GC-MS. Disarmed Hyoscymouw tumefaciens strain C58C1 harbor- ing both pBMI and Agrobacterium rhizogenes Ri plasmid pRiA4, containing a single pmt gene, were used for plant transformation 16, Enhanced scopolamine production in Hyoscyamus plants and hairy root cultures.

Cell cultures deriving from haiyr offer a fascinating tool to study plant metabolic pathways and offer large scale production systems for valuable compounds — commercial examples include compounds such as paclitaxel.

Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures.

Both producing plant species Root tips were thawed in a following way unless otherwise described. It is concluded that, as in the case of transgenics that include The current study provides an effective approach for commer- both pmt and h6h in H. Even though earlier reports of cryopreservation of hairy roots of certain plant species have been published, so far there exists no methods reported for cryostorage of hairy roots of Hyoscyamus sp.

The transgenic lines showed pmt transcripts at various levels. Although genetic and metabolic stability has often been connected to transgenic hairy roots, there are only few reports on how a very long-term subculturing effects on the mjticus capacity of hairy roots.

Are the high scopolamine levels associated only with h6h This report on engineering pmt and h6h simultaneously into single-gene overexpression, or with a coordinative effect of hyoscymlus scopolamine-producing plant species results mticus significant en- pmt and h6h transgenes? Introduction Plant Cell Cultures Plants offer an enormous potential for humans in applications such as novel drugs, biopolymers, high-value chemical compounds, food and feed.

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The tropane alkaloids from the culture medium were extracted mutiucs from 2 ml medium. Here, we present an easy method for cryopreservation of H. Chemistry and Pharma- The statistical analysis was performed with the computing environment R R Core Team, As described 16 years ago, the hairy roots have preserved their light color and fast growth rate, multiplying the biomass 65—67 fold in 28 days.

Remember me on this computer. Epigenetic aspects of somaclonal variation in plants. Transgenic crops for improved pharmaceutical products. The pXI was isolated from E. Overexpression of one enzyme obtained from the Second Military Medical University Shang- often renders subsequent reactions more rate-limiting, mkticus thus hai, China and germinated into plants.

All of the P and T nol. Only few reports show the accumulation of metabolites produced by hairy roots during a long subculturing.

Cryopreservation of hairy root cultures of Maesa lanceolata and Medicago truncatula. Biotechnology relies to a great part on working with cell cultures hairh continues to offer a future beyond depletion of natural resources.

Skip to main content. Engineering biosynthesis of high-value compounds in photosynthetic organisms. Plant alka- loids constitute the largest groups of natural products, providing ratio of scopolamine to hyoscyamine in scopolamine-producing cultured roots 9. We will be provided with an authorization token please note: They per sample were denatured and separated on a 1.

About derived from transformation T lines by using the same PCR mg of fresh roots 3 cm in length were inoculated into ml conditions for the detection of the h6h gene. Total RNA was isolated from separately the dark. Comparison of plant-based expression platforms for the heterologous production of geraniol. The best line T3 produced